Life Sensors是一家為藥物發現和研究市場提供方法和建立可行性技術方法的生物技術公司。該公司為泛素和泛素蛋白酶體通路研究提供創新性的研究工具而占據世界。其原核和真核蛋白表達系統SUMOpro 和SUMOstar 已被世界范圍內的學術、政府研究機構和制藥、生物技術公司廣泛應用。

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SilenciX Ubiquitin Pathway Knockdown Cell Lines The first generation of RNAi tools, siRNA and basic plasmids expressing shRNA, reveal certain limitations and off-target effects. Furthermore, successful RNAi experiments currently involve significant optimization requiring additional resources. SilenciX® stable knockdown cell lines have been developed based on a unique siRNA delivery system developed by tebu-bio Laboratories* providing improved efficacy and stability of RNAi knockdown. Based on EBV-replication, this loss-of-function model ensures chromosome-like replication of the expression vector in a limited and constant number without integration in the genome. This eliminates off-target effects and saturation of the RNAi machinery resulting in specific and homogeneous knockdown of the desired target. LifeSensors is offering SilenciX®, gene-specific ready-to-use knock-down cell lines ‘silencing’ deubiquitylases （DUBs） and other proteins of interest, including DNA repair-related genes, providing invaluable tools for loss-of-function approaches. *SilenciX® is a registered trademark of tebu-bio; technology licensed from the CEA. SILENCIX® KNOCK-DOWN CELL LINES - DEUBIQUITYLASES （DUBS） SILENCIX® KNOCK-DOWN CELL LINES - DNA DAMAGE AND REPAIR SILENCIX® KNOCK-DOWN CUSTOM CELL LINES

Custom Ubiquitinated Peptides Synthesis of custom monoubiquitinated peptides designed from your protein of interest Choose the protein. . . Choose the ubiquitination site. . . We’ll deliver a monoubiquitinated peptide! LifeSensors can provide custom monubiquitinated peptides from the sequence of your choosing. lsopeptide-linked ubiquitinated peptide reagents are produced using peptide synthesis technologies. Stepwise solid-phase synthesis and fragment condensation / chemical ligation approaches are employed in the synthesis of the ubiquitinated peptides. By utilizing these complementary methodologies for peptide synthesis, a large variety of native and labelled 'isopeptide-linked' ubiquitinated peptides can be generated for a variety of applications including: •    DUB substrates for screening •    Antigens for generation of site-specific ubiquitin antibodies •    Structural studies of ubiquitin-binding domain recognition In contrast to traditional DUB substrates, like ubiquitin-AMC and ubiquitin-rhodamine, which measure cleavage of amide bonds, utilization of a monoubiquitinated peptide as a DUB substrate measures cleavage of an isopeptide bond and allows determination of the influence of sequence proximal to the ubiquitinated lysine on specificity. Profiling activity of a panel of DUBs against a monoubiquitinated histone H2B peptide and a monoubiquitinated PTEN peptide reveals interesting differences in specificity:   Assessment of DUB activity using monoubiquitinated peptides as a substrates. 2µM monoubiquitinated histone H2B peptide （red）, 2µM monoubiquitinated PTEN peptide （blue）, and 2µM ubiquitin-rhodamine were incubated with 50nM of each indicated DUB for 30 minutes at 37ºC. Isopeptidase cleavage of ubiquitin from the monoubiquitinated peptides was assessed by LC-MS and is represented as the percent substrate cleaved. Cleavage of ubiquitin-rhodamine was measured as the increase in rhodamine fluorescence at 535nm and is represented as the rate （RFU/sec） relative to USP2c. Monoubiquitinated PTEN and histone H2B peptides are available from stock. Length: peptides of almost any length can be synthesized Quantity: µg to gram quantities Purity: choose purity levels from 80-98% Fluorescent modifications: examples include fluorescein, TAMRA, AlexaFluor®, or FRET pairs Affinity tags: examples include biotin, His6, FLAG®, V5 Ubiquitin options: peptides can be synthesized with wild-type or mutant ubiquitins, including lysine to arginine substitutions or unnatural amino acids Contact us at info@lifesensors.com or call 610-644-8845 for a quotation or more information.